King K. Rool is trying to destroy DK Isles with a large laser called the Blast-O-Matic, but it malfunctions after a crash that puts his floating, mechanical hind face-to-face with DK Isles. To buy some time, he captures Donkey Kong's friends and locks them up, and then steals Donkey Kong's precious hoard of Golden Bananas. As Donkey Kong frees his fellow apes, they set off to recover the bananas and defeat King K. Rool and his army of Kremlings and other evil creatures.